Equipped with 488 and 640 nm solidstate lasers, these flow cytometers analyze up to six parameters fsc, ssc and four fluorescences. Bd life sciences diagnostic systems qcpi manuals bd. Some of our leading manufacturers include agilent, hp, waters, ge, amersham, dionex and shimazdzu. Bd pyxis medstation es, automated medication dispensing. The accuri csampler is an optional accessory for the c6 flow cytometer that. Operate the instrument as described in this instruction manual. Mesf values determined using thermo scientific cytocal multifluor plus violet beads. If you have questions or require further clarification, see the bd accuri c6 flow cytometer instrument manual or contact accuri technical support. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Rhosonics sdm series manual manual 65 pages sciex pace mdq plus site planning manual site planning manual 18 pages maynuo. Our hplc systems such as the agilent 1100 and 1200 series, akta fplcs, and.
Bd accuri c6 flow cytometer performance specifications summary tools. The bd pyxis medstation es is an automated medication dispensing system supporting decentralized medication management. Anyone have experience with the new bd accuri c6 plus. In addition, c6 has a flow metering system for automatic calculation of absolute cell counts. The mean levels of h2ax proteins expressed in cells were measured on a bd accuri c6 plus system bd biosciences, san jose, ca, usa and presented as the mean values of the intensities of fluorescence emitted by the excited alexa fluor 488 dye that were conjugated with the secondary antibodies. Biolegends legendplex kits are provided with predefined panels, ranging from 3 to specificities, or customers can mix and match any subset within each predefined panel using our mix and match system. It has great sensitivity and broad dynamic range of 4000 qtrap, making it an optimum system for srmmrm types of experiments.
Bd accuri c6 plus panel builder user s guides and manuals. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas vestibulum tortor quam. All users are responsible for obtaining the proper approval and training before the use of the bd accuri c6 flow cytometer. Flowjo portal provides an improved management interface to site administrators. Designed from the ground up to be compact and easy to use, the bd accuri c6 flow cytometer fits in any lab and is readily accessible. To get your question to the right person quickly, please provide the.
Bd accuri c6 with single tube loader protocol this protocol contains instructions for setting up an accuri c6 with tube loader. Bd accuri c6 cytometer with automated, multisamplerflow cytometer. We dont know what we are doing wrong, nor do we know how to fix it. Accuri c6 flow cytometry analyzer the satelite facility at 348 burrill hall dr. Bd accuri c6 software user guide manual 782009501 rev0 iii f. Bd has discontinued selling the bd accuri c6 flow cytometer as of december 31, 2016. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. David kranzs lab click link for campus map is equipped with an accuri c6 4color flow cytometry analyzer. This user manual contains important information on using the egg incubator please retain this user manual for future.
Assemble the computer according to the manufacturers instructions. I keep hearing that the bd accuri c6 flow cytometer is reliable. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. View online quick reference manual for bd facscanto ii measuring instruments or simply click download button to examine the bd facscanto ii guidelines offline on your desktop or laptop computer. Standard fluorochrome in use are identical to facs calibur. Flow cytometry is a laser based technology used to count and sort cells, detect biomarkers and engineer proteins, by suspending cells in a stream of fluid and passing them by an electronic detection system. Accuri c6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to facscalibur. Bd accuri c6 plus software files can be exported in fcs 3. Bd facscanto ii measuring instruments user manual manual. Determination of sperm concentration using flow cytometry. Download user manual manual of bd facscanto ii laboratory equipment, measuring instruments for free or view it online on. Accuri cytometers c6 flow cytometer community, manuals. All users are responsible for the proper use, according to defined protocol, when using the bd accuri c6 flow cytometer.
The accuri c6 is a great work horse for the research lab that requires fourcolor analysis. The bd pyxis es platform offers enterprisewise medication management through integration with hit systems. Nihon kohden automated hematology analyzer mek6510k. Flow cytometer 204 pages laboratory equipment bd facscan instructions 2 pages. The bd accuri c6 plus personal flow cytometer is the newest generation of the bd accuri platform. Fast and accurate prediction of positive and negative. The data we obtain from the c6 tell us that this instrument is, just as, if not more, sensitive as more expensive instruments and maintenance is so easy. Bd csampler software user guide iv 782009901 rev2 table of figures figure 11. If you are the first user of the day, you can run for 15 minutes. We offer an assortment of refurbished and used liquid chromatography solutions ranging from hplc, lcms to fplc. Accuri c6 is an easy to use flow cytometer which can be connected to an autosampler 48 and 96 well, and 24 tube rack, we do not have one. Bd accuri c6 flow cytometer instrument manual 782001801 rev2 1 the bd accuri c6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research.
It is the responsibility of the user buyer to install and maintain antivirus software on the computer. The system includes blue and red lasers, four color detectors, and both forward and side scatter detectors plus software that is so intuitive that. Service for bd accuri c6 systems and their related options will continue until december 31 st, 2021. Bd accuri c6 plus software features an intuitive user interface. Accuri technical support for assistance with other operating systems. Bd accuri c6 plus cell analyzer your personal flow cytometer bd facscelesta cell analyzer the power of the core on your benchtop. Laboratory equipment bd accuri c6 instrument manual.
Open the lid of the bd accuri c6 and locate the holes used to mount the bd csampler figure 4. Bd accuri c6 flow cytometer quick set up guide please follow these directions for setting up your bd accuri c6 flow cytometer. Service for bd accuri c6 systems and their related options will continue until april 15, 2021. Beckman l870m ultracentrifuge shared instrumentation. The instrument was validated and maintained daily according to the user manual. Bd biosciences bd accuri c6 plus community, manuals and. A flowjo portal site license is a user based license management system for a group or institution.
The absciex 4000 qtrap is a triple quadruple qqq instrument, specialized in selected reaction monitors srmmultiple reaction monitor mrm for absolute quantification of molecules in a complex mixture. Can anyone please suggest one which will be the best choice between bd acuuri c6 or bd facsverse. For a limited time only, purchase a bd accuri c6 plus or a bd accuri c6 plus and csampler plus in combination with an extra year of warranty for a promotional price. The accuri c6 is equipped with two excitation lasers. Easytouse software with automated qc and application templates bd accuri c6 plus software features an intuitive user interface. Administrators can easily invite users, manage registrations, and customize their unique site. Plus the data produced by the c6 can be smoothly analyzed by thirdparty software such as flowjo.
Designed from the ground up to be compact and easy to use, the bd accuri c6. Follow the installation instructions in the wizard. Data was generated on either the bd accuri c6 plus or the bd accuri. Accuric6 flow cytometry core facility university of bergen. Bd accuri c6 flow cytometer instrument manual 7820018 rev4 1 1 introduction the bd accuri c6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Biolegends legendplex is a beadbased immunoassay that uses the principles of sandwich elisas to quantify soluble analytes using a flow cytometer. Bd accuri c6 flow cytometer instrument manual bd biosciences. Bd accuri c6 plus compensation calculator microsoft excel bd accuri c6 plus fluorochromedye reference chart. Accuri c6 maintenance cleaning and decontamination alternatives. Hold the bd csampler module with both hands and locate the tab in the slot on the back of the bd accuri c6 figure 5.
All reagents and kits are compatible with both the bd accuri c6 plus and bd accuri c6 flow cytometer systems. Request a quote or demo on a bd accuri c6 plus system. It helps clinicians safely and efficiently dispense the right medications, for the right patients at the right time. Platforms referred to as bd accuri represent both the bd accuri c6 plus and bd accuri c6. After purchase this year of accuri cytometers, bd biosciences commercializes two new bench top analyzers. Accuri c6 is a digital pc based bench top analyser, 2 laser system. Learn about bd life sciences diagnostic systems qcpi manuals. After receiving instrument training from the flow cytometry facility staff, users are granted 247 access to the lab advanced booking is required. Developed equivalency testing procedure, authored and performed procedures for bacterial stock growth and qualification including stock enumeration using bd accuri c6 plus flow cytometer.
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